Review



stat5b plasmid  (OriGene)


Bioz Verified Symbol OriGene is a verified supplier
Bioz Manufacturer Symbol OriGene manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 91
      Buy from Supplier

    Structured Review

    OriGene stat5b plasmid
    Figure 2. JAK3 Y841 and the homologous JAK1 Y894 are critical for the activation of <t>STAT5B.</t> (a) Hek293 cells were transfected with 10 µg WT (lane a) or mutant JAK3 plasmids (lanes b–i) and incubated for 48 h at 37 ◦C. Cells were harvested after 48 h, immunoprecipitated for JAK3 and Western blotted for total pY. Shown is a representative blot from n = 2 independent experiments. Individual points for replicate experiments are provided in Figure S2a. (b) Hek293 cells were transfected with 2 µg of WT (lane a) or mutant JAK3 plasmids (lanes b–i) with 2 µg of STAT5B plasmids (lanes b–i) and harvested after 48 h at 37 ◦C. Western blot was performed using anti-pYSTAT5 and reblotted for total STAT5 to confirm the equal protein expression and loading of STAT5B. Shown is a representative blot from n = 3 independent experiments. (c) Densitometry analysis using LI-COR Image Studio Digits
    Stat5b Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat5b+plasmid/STAT5B+(NM_012448)+Human+Tagged+ORF+Clone/pm37569303-277-23-25
    Average 91 stars, based on 1 article reviews
    stat5b plasmid - by Bioz Stars, 2026-11
    91/100 stars

    Images

    1) Product Images from "JAK3 Y841 Autophosphorylation Is Critical for STAT5B Activation, Kinase Domain Stability and Dimer Formation."

    Article Title: JAK3 Y841 Autophosphorylation Is Critical for STAT5B Activation, Kinase Domain Stability and Dimer Formation.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms241511928

    Figure 2. JAK3 Y841 and the homologous JAK1 Y894 are critical for the activation of STAT5B. (a) Hek293 cells were transfected with 10 µg WT (lane a) or mutant JAK3 plasmids (lanes b–i) and incubated for 48 h at 37 ◦C. Cells were harvested after 48 h, immunoprecipitated for JAK3 and Western blotted for total pY. Shown is a representative blot from n = 2 independent experiments. Individual points for replicate experiments are provided in Figure S2a. (b) Hek293 cells were transfected with 2 µg of WT (lane a) or mutant JAK3 plasmids (lanes b–i) with 2 µg of STAT5B plasmids (lanes b–i) and harvested after 48 h at 37 ◦C. Western blot was performed using anti-pYSTAT5 and reblotted for total STAT5 to confirm the equal protein expression and loading of STAT5B. Shown is a representative blot from n = 3 independent experiments. (c) Densitometry analysis using LI-COR Image Studio Digits
    Figure Legend Snippet: Figure 2. JAK3 Y841 and the homologous JAK1 Y894 are critical for the activation of STAT5B. (a) Hek293 cells were transfected with 10 µg WT (lane a) or mutant JAK3 plasmids (lanes b–i) and incubated for 48 h at 37 ◦C. Cells were harvested after 48 h, immunoprecipitated for JAK3 and Western blotted for total pY. Shown is a representative blot from n = 2 independent experiments. Individual points for replicate experiments are provided in Figure S2a. (b) Hek293 cells were transfected with 2 µg of WT (lane a) or mutant JAK3 plasmids (lanes b–i) with 2 µg of STAT5B plasmids (lanes b–i) and harvested after 48 h at 37 ◦C. Western blot was performed using anti-pYSTAT5 and reblotted for total STAT5 to confirm the equal protein expression and loading of STAT5B. Shown is a representative blot from n = 3 independent experiments. (c) Densitometry analysis using LI-COR Image Studio Digits

    Techniques Used: Activation Assay, Transfection, Mutagenesis, Incubation, Immunoprecipitation, Western Blot, Expressing

    Related Articles

    Activation Assay:

    Article Title: JAK3 Y841 Autophosphorylation Is Critical for STAT5B Activation, Kinase Domain Stability and Dimer Formation.
    Article Snippet: .. For the JAK3/STAT5 activation studies, HEK293 cells were transfected with 2 μg of pcDNA3.1/human JAK3 plasmid [19] alone or with 2 μg of STAT5B plasmid (OriGene, Rockville, MD, USA) per confluent in 10 cm dishes. ..

    Article Title: JAK3 Y841 Autophosphorylation Is Critical for STAT5B Activation, Kinase Domain Stability and Dimer Formation
    Article Snippet: .. For the JAK3/STAT5 activation studies, HEK293 cells were transfected with 2 μg of pcDNA3.1/human JAK3 plasmid [ ] alone or with 2 μg of STAT5B plasmid (OriGene, Rockville, MD, USA) per confluent in 10 cm dishes. ..

    Transfection:

    Article Title: JAK3 Y841 Autophosphorylation Is Critical for STAT5B Activation, Kinase Domain Stability and Dimer Formation.
    Article Snippet: .. For the JAK3/STAT5 activation studies, HEK293 cells were transfected with 2 μg of pcDNA3.1/human JAK3 plasmid [19] alone or with 2 μg of STAT5B plasmid (OriGene, Rockville, MD, USA) per confluent in 10 cm dishes. ..

    Article Title: JAK3 Y841 Autophosphorylation Is Critical for STAT5B Activation, Kinase Domain Stability and Dimer Formation
    Article Snippet: .. For the JAK3/STAT5 activation studies, HEK293 cells were transfected with 2 μg of pcDNA3.1/human JAK3 plasmid [ ] alone or with 2 μg of STAT5B plasmid (OriGene, Rockville, MD, USA) per confluent in 10 cm dishes. ..

    Plasmid Preparation:

    Article Title: JAK3 Y841 Autophosphorylation Is Critical for STAT5B Activation, Kinase Domain Stability and Dimer Formation.
    Article Snippet: .. For the JAK3/STAT5 activation studies, HEK293 cells were transfected with 2 μg of pcDNA3.1/human JAK3 plasmid [19] alone or with 2 μg of STAT5B plasmid (OriGene, Rockville, MD, USA) per confluent in 10 cm dishes. ..

    Article Title: JAK3 Y841 Autophosphorylation Is Critical for STAT5B Activation, Kinase Domain Stability and Dimer Formation
    Article Snippet: .. For the JAK3/STAT5 activation studies, HEK293 cells were transfected with 2 μg of pcDNA3.1/human JAK3 plasmid [ ] alone or with 2 μg of STAT5B plasmid (OriGene, Rockville, MD, USA) per confluent in 10 cm dishes. ..



    Similar Products

    n terminal flag tag  (Sino Biological)
    90
    Sino Biological n terminal flag tag
    N Terminal Flag Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat5b+plasmid/Mouse+STAT5b+Gene+ORF+cDNA+clone+expression+plasmid%2C+N-Flag+tag/bio_rxiv__2022__02__14__480424-29-7-13
    Average 90 stars, based on 1 article reviews
    n terminal flag tag - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    pad cmv stat5b cmv gfp plasmid  (Addgene inc)
    91
    Addgene inc pad cmv stat5b cmv gfp plasmid
    Pad Cmv Stat5b Cmv Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat5b+plasmid/pAD-CMV-STAT5B-CMV-GFP+(Plasmid+%2383257)/pm37595871-210-1-6
    Average 91 stars, based on 1 article reviews
    pad cmv stat5b cmv gfp plasmid - by Bioz Stars, 2026-11
    91/100 stars
    		  Buy from Supplier
    stat5b plasmid  (OriGene)
    91
    OriGene stat5b plasmid
    Figure 2. JAK3 Y841 and the homologous JAK1 Y894 are critical for the activation of <t>STAT5B.</t> (a) Hek293 cells were transfected with 10 µg WT (lane a) or mutant JAK3 plasmids (lanes b–i) and incubated for 48 h at 37 ◦C. Cells were harvested after 48 h, immunoprecipitated for JAK3 and Western blotted for total pY. Shown is a representative blot from n = 2 independent experiments. Individual points for replicate experiments are provided in Figure S2a. (b) Hek293 cells were transfected with 2 µg of WT (lane a) or mutant JAK3 plasmids (lanes b–i) with 2 µg of STAT5B plasmids (lanes b–i) and harvested after 48 h at 37 ◦C. Western blot was performed using anti-pYSTAT5 and reblotted for total STAT5 to confirm the equal protein expression and loading of STAT5B. Shown is a representative blot from n = 3 independent experiments. (c) Densitometry analysis using LI-COR Image Studio Digits
    Stat5b Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat5b+plasmid/STAT5B+(NM_012448)+Human+Tagged+ORF+Clone/pm37569303-277-23-25
    Average 91 stars, based on 1 article reviews
    stat5b plasmid - by Bioz Stars, 2026-11
    91/100 stars
    		  Buy from Supplier
    stat5b double nickase plasmid  (Santa Cruz Biotechnology)
    91
    Santa Cruz Biotechnology stat5b double nickase plasmid
    Figure 2. JAK3 Y841 and the homologous JAK1 Y894 are critical for the activation of <t>STAT5B.</t> (a) Hek293 cells were transfected with 10 µg WT (lane a) or mutant JAK3 plasmids (lanes b–i) and incubated for 48 h at 37 ◦C. Cells were harvested after 48 h, immunoprecipitated for JAK3 and Western blotted for total pY. Shown is a representative blot from n = 2 independent experiments. Individual points for replicate experiments are provided in Figure S2a. (b) Hek293 cells were transfected with 2 µg of WT (lane a) or mutant JAK3 plasmids (lanes b–i) with 2 µg of STAT5B plasmids (lanes b–i) and harvested after 48 h at 37 ◦C. Western blot was performed using anti-pYSTAT5 and reblotted for total STAT5 to confirm the equal protein expression and loading of STAT5B. Shown is a representative blot from n = 3 independent experiments. (c) Densitometry analysis using LI-COR Image Studio Digits
    Stat5b Double Nickase Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat5b+plasmid/Stat5b+Double+Nickase+Plasmid/pmc10245388-188-9-22
    Average 91 stars, based on 1 article reviews
    stat5b double nickase plasmid - by Bioz Stars, 2026-11
    91/100 stars
    		  Buy from Supplier
    stat5b sc  (Santa Cruz Biotechnology)
    86
    Santa Cruz Biotechnology stat5b sc
    Figure 2. JAK3 Y841 and the homologous JAK1 Y894 are critical for the activation of <t>STAT5B.</t> (a) Hek293 cells were transfected with 10 µg WT (lane a) or mutant JAK3 plasmids (lanes b–i) and incubated for 48 h at 37 ◦C. Cells were harvested after 48 h, immunoprecipitated for JAK3 and Western blotted for total pY. Shown is a representative blot from n = 2 independent experiments. Individual points for replicate experiments are provided in Figure S2a. (b) Hek293 cells were transfected with 2 µg of WT (lane a) or mutant JAK3 plasmids (lanes b–i) with 2 µg of STAT5B plasmids (lanes b–i) and harvested after 48 h at 37 ◦C. Western blot was performed using anti-pYSTAT5 and reblotted for total STAT5 to confirm the equal protein expression and loading of STAT5B. Shown is a representative blot from n = 3 independent experiments. (c) Densitometry analysis using LI-COR Image Studio Digits
    Stat5b Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat5b+plasmid/Stat5b+HDR+Plasmid/pmc06678910__cancers___11___00929___s001-72-11-7
    Average 86 stars, based on 1 article reviews
    stat5b sc - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier
    plasmid encoding with stat5b gene (pcmv6-ac-gfp-stat5b)  (OriGene)
    90
    OriGene plasmid encoding with stat5b gene (pcmv6-ac-gfp-stat5b)
    Figure 2. JAK3 Y841 and the homologous JAK1 Y894 are critical for the activation of <t>STAT5B.</t> (a) Hek293 cells were transfected with 10 µg WT (lane a) or mutant JAK3 plasmids (lanes b–i) and incubated for 48 h at 37 ◦C. Cells were harvested after 48 h, immunoprecipitated for JAK3 and Western blotted for total pY. Shown is a representative blot from n = 2 independent experiments. Individual points for replicate experiments are provided in Figure S2a. (b) Hek293 cells were transfected with 2 µg of WT (lane a) or mutant JAK3 plasmids (lanes b–i) with 2 µg of STAT5B plasmids (lanes b–i) and harvested after 48 h at 37 ◦C. Western blot was performed using anti-pYSTAT5 and reblotted for total STAT5 to confirm the equal protein expression and loading of STAT5B. Shown is a representative blot from n = 3 independent experiments. (c) Densitometry analysis using LI-COR Image Studio Digits
    Plasmid Encoding With Stat5b Gene (Pcmv6 Ac Gfp Stat5b), supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat5b+plasmid/stat3+cdna/pm27853239-143-1-13
    Average 90 stars, based on 1 article reviews
    plasmid encoding with stat5b gene (pcmv6-ac-gfp-stat5b) - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 2. JAK3 Y841 and the homologous JAK1 Y894 are critical for the activation of STAT5B. (a) Hek293 cells were transfected with 10 µg WT (lane a) or mutant JAK3 plasmids (lanes b–i) and incubated for 48 h at 37 ◦C. Cells were harvested after 48 h, immunoprecipitated for JAK3 and Western blotted for total pY. Shown is a representative blot from n = 2 independent experiments. Individual points for replicate experiments are provided in Figure S2a. (b) Hek293 cells were transfected with 2 µg of WT (lane a) or mutant JAK3 plasmids (lanes b–i) with 2 µg of STAT5B plasmids (lanes b–i) and harvested after 48 h at 37 ◦C. Western blot was performed using anti-pYSTAT5 and reblotted for total STAT5 to confirm the equal protein expression and loading of STAT5B. Shown is a representative blot from n = 3 independent experiments. (c) Densitometry analysis using LI-COR Image Studio Digits

    Journal: International journal of molecular sciences

    Article Title: JAK3 Y841 Autophosphorylation Is Critical for STAT5B Activation, Kinase Domain Stability and Dimer Formation.

    doi: 10.3390/ijms241511928

    Figure Lengend Snippet: Figure 2. JAK3 Y841 and the homologous JAK1 Y894 are critical for the activation of STAT5B. (a) Hek293 cells were transfected with 10 µg WT (lane a) or mutant JAK3 plasmids (lanes b–i) and incubated for 48 h at 37 ◦C. Cells were harvested after 48 h, immunoprecipitated for JAK3 and Western blotted for total pY. Shown is a representative blot from n = 2 independent experiments. Individual points for replicate experiments are provided in Figure S2a. (b) Hek293 cells were transfected with 2 µg of WT (lane a) or mutant JAK3 plasmids (lanes b–i) with 2 µg of STAT5B plasmids (lanes b–i) and harvested after 48 h at 37 ◦C. Western blot was performed using anti-pYSTAT5 and reblotted for total STAT5 to confirm the equal protein expression and loading of STAT5B. Shown is a representative blot from n = 3 independent experiments. (c) Densitometry analysis using LI-COR Image Studio Digits

    Article Snippet: For the JAK3/STAT5 activation studies, HEK293 cells were transfected with 2 μg of pcDNA3.1/human JAK3 plasmid [19] alone or with 2 μg of STAT5B plasmid (OriGene, Rockville, MD, USA) per confluent in 10 cm dishes.

    Techniques: Activation Assay, Transfection, Mutagenesis, Incubation, Immunoprecipitation, Western Blot, Expressing